This examination was embraced to explore the appropriation of etiological specialists and antimicrobial opposition design among the blood culture-positive instances of neonatal sepsis admitted to a dimension III neonatal consideration unit in a showing medical clinic Kolkata, Eastern India for the time of 4 years (2014 – 2017). Blood culture was accomplished for all infants conceded with clinical sepsis or with positive sepsis screen via mechanized BACTEC 9050 framework. In positive cases, distinguishing proof of creature was done trailed by antimicrobial affectability testing by Kirby Bauer plate dissemination strategy utilizing Clinical Research Center Standard Organization Rules. Multi-sedate obstruction (MDR) was characterized as the nearness of protection from at any rate three of the five anti-microbial gatherings if there should arise an occurrence of gram-negative microscopic organisms. A chi-squared test was performed to test the relationship between various gatherings. Out of 4781 instances of clinical neonatal sepsis, 415 (8.68%) were culture positive. In 240 (69%) cases, the pathogens had a place with the family Enterobacteriaceae. Gram-positive cocci represent 38 (11%) instances of positive blood culture cases. Klebsiella is the preeminent irresistible operator with positive in 187(54%) of all bacterial segregates.